Journal: Heliyon

Article Title: Liraglutide ameliorates TAC-induced cardiac hypertrophy and heart failure by upregulating expression level of ANP expression

doi: 10.1016/j.heliyon.2024.e32229

Figure Lengend Snippet: Liraglutide alleviates transverse aortic constriction (TAC)-induced cardiac hypertrophy and heart failure, accompanied by substantial upregulation of ANP expression and release. A, Representative images of gross morphology of hearts (scale bar is 2 mm), hematoxylin-eosin (HE) staining (scale bar is 1 mm), wheat germ agglutinin (WGA) staining (scale bar is 50 μm), heart weight/tibia length (HW/TL) ratio, heart weight/body weight (HW/BW) ratio, and quantification of the cross-sectional area (CSA) of the hearts from the Sham, TAC, and TAC + Liraglutide (Lira) groups at 4 weeks after TAC (n = 5–6 per group). B, Echocardiographic analyses of cardiac function, including ejection fraction (EF), fractional shortening (FS), left ventricular internal diameter end systole (LVIDs) and left ventricular internal diameter end diastole (LVIDd) of the hearts from indicated groups (n = 5–6 per group). C, Western blot analyses and quantification of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and myosin heavy polypeptide 7 (MYH7) protein levels of the hearts form indicated groups (n = 6 per group). D, Real-time quantitative polymerase chain reaction (RT-qPCR) analyses and quantification of ANP, BNP, and MYH7 mRNA levels of the hearts from indicated groups (n = 5 per group). E, ELISA analyses of serum ANP levels of the mice from indicated groups (n = 5 per group). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ANP levels in mouse serum were quantified using the ANP ELISA kit (NBP2-66733, NOVUS) following the manufacturer's guidelines.

Techniques: Expressing, Staining, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Circadian disruption during fetal development promotes pathological cardiac remodeling in male mice

doi: 10.1016/j.isci.2024.109008

Figure Lengend Snippet:

Article Snippet: Mouse Brain Natriuretic Peptide (BNP) ELISA test Kit , Cloud-Clone , Cat# SEA541Mu.

Techniques: Recombinant, Virus, Fluorescence, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software